ctaB [2019-06-27 15:16:16]

heme O synthase (major enzyme)

Locus: BSU14880

Isoelectric point: 9.57

Molecular weight: 33.79 kDa

Protein length: 305 aa

Gene length: 918 bp

Function: heme biosynthesis

Product: heme O synthase (major enzyme)

Essential: no

E.C.: 2.5.1.-

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of heme/ siroheme
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,559,309 1,560,226

Phenotypes of a mutant

• inactivation of ctaB facilitates without a wall (due to reduction of oxidative stress) PubMed

The protein

Protein family

• ubiA prenyltransferase family, Protoheme IX farnesyltransferase subfamily (according to Swiss-Prot)

Paralogous protein(s)

• CtaO

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ctaB-ctaC-ctaD-ctaE-ctaF-ctaG
  Description: PubMed

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • Abh: repression, synergistic repression with AbrB, in Abh regulon
  • CcpA: repression, in CcpA regulon
  • AbrB: repression, synergistic repression with Abh, in AbrB regulon

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE14880 (ctaB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACGTAAACCTCCCTTA, downstream forward: _UP4_TAAAAAATAGATATGATGCT
• BKK14880 (ctaB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAACGTAAACCTCCCTTA, downstream forward: _UP4_TAAAAAATAGATATGATGCT

References

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